MYCOLab Ai Logo - gold and army green

Bioactivity and toxicity of polysaccharides derived from the phytopathogenic mushroom Ganoderma orbiforme cultured in a bioreactor

Summary

Scientists studied a mushroom called Ganoderma orbiforme that normally harms oil palm trees to see if it could be useful as medicine. They grew the mushroom in a special bioreactor and extracted beneficial substances called polysaccharides. These substances showed promise as natural antibiotics and antioxidants while being safe for use based on safety testing with fish embryos.

Background

Ganoderma orbiforme is known as a phytopathogenic agent causing basal stem rot in oil palm, but its biotechnological potential remains largely unexplored. Medicinal mushrooms, particularly Ganoderma species, have been valued in traditional Asian medicine for their bioactive compounds including polysaccharides. This study investigates the potential therapeutic applications of G. orbiforme while ensuring safety assessment.

Objective

To explore the growth profile of G. orbiforme in an Air-L-Shaped Bioreactor, evaluate the antibacterial and antioxidant activities of its extracellular and intracellular polysaccharides, and assess their toxicity using zebrafish embryo toxicity assays.

Results

Biomass peaked at 2.53 g/L with EPS and IPS yields of 0.15 g/L and 0.06 g/L respectively. EPS demonstrated superior antibacterial activity with MIC values of 5 mg/mL against Streptomyces griseus and Staphylococcus epidermidis. Both EPS and IPS showed strong antioxidant capabilities with IC50 values of 15.59 mg/mL and 26.85 mg/mL in DPPH assays. LC50 values of 1.88 mg/mL and 1.56 mg/mL demonstrated harmless toxicity profiles.

Conclusion

G. orbiforme exhibits significant antibacterial and antioxidant potential through its polysaccharides while demonstrating safety in zebrafish embryo toxicity assays. The study underscores the importance of bioprospecting phytopathogens for therapeutic applications in oil palm-producing countries. These findings align with sustainable development goals for health and environmental protection.
  • Published in:Scientific Reports,
  • Study Type:Experimental Research,
  • Source: PMID: 41272120, DOI: 10.1038/s41598-025-25352-2
Scroll to Top